As healthcare systems continue to combat COVID-19, and the ongoing risk posed by Multidrug-Resistant Organisms (MDROs), ultraviolet (UV) room disinfection can be a valuable tool for enhanced environmental hygiene and surface disinfection. In implementing UV room disinfection, it is key for healthcare professionals to ensure a specific UV device is truly effective against SARS-CoV-2 and its variants, as well as a broad spectrum of high-risk microorganisms including C. difficile spores, MRSA, Candida auris and CRE/VRE. This program will review (1) the basics of how UV room disinfection devices inactivate microorganisms (2) the germicidal UV dose delivery required to achieve broad spectrum efficacy and (3) the basics for evaluating and decoding UV device manufacturers’ claims and support, which can vary greatly.