This session was presented at the November 2012 Managing the Spectrum of Infection Prevention in Acute and Ambulatory Settings symposium held in Atlanta. The focus of this session, C. difficile and MRSA: Recent Developments in Epidemiology, Diagnosis, Treatment and Prevention, was on two organisms that have a major impact on infection prevention measures: Methicillin-Resistant Staphylococcus aureus (MRSA) and Clostridium difficile (C. diff). A brief review of the epidemiology of both healthcare-acquired and community-acquired MRSA and the relationship between transmission of community-acquired and healthcare-acquired MRSA will be explained. The genetic elements of MRSA associated with antimicrobial resistance will be discussed. Vertical and horizontal transmission measures for prevention of MRSA will be described. Dr. Salgado will then provide an update on Clostridium difficile infections (CDI). Emerging changes in CDI will include: epidemiology issues; risk factor; pathogenicity of the NAP 1 epidemic strain of Clostridium difficile; an improved diagnostic testing technique and prevention measures for CDI. This videotaped presentation will be enhanced with synchronized audio recordings and PowerPoint slides. The participant will complete test questions and a course evaluation form to measure the value of this learning experience.